讨论了耐氧两歧双歧杆菌在小瓶培养、发酵罐间歇培养、及流加培养的生长及代谢特性,探讨了其高密度培养的途径。在发酵罐流加培养试验中,通过流加碱和流加基质培养,使双歧杆菌生长的OD600值达到1.67,菌体浓度约为6.3×1010个/mL,乳酸浓度达到620mg/100mL,并使双歧杆菌生长的周期明显延长。
The growth and metabolic characteristics of Bifidobacterium bifidum in vial culture,batch fermentation or fed-batch fermentation were discussed respectively.The approaches to realize high cell density culture of B.bifidum was also studied.In fed-batch fermentation by the feeding of alkali and basic precursors,the OD600 and cell density of B.bifidum reached 1.67 and 6.3×1010 cells/ml respectively,and the lactic acid concentration was 620 mg/100 ml.Besides,the growth cycle of B.bifidum was prolonged obviously... |