目的：采用大鼠30％Ⅲ°烫伤模型，观察：①大鼠烧伤后及不同时机切痂后血循环中和脾脏、肠系膜淋巴结内Th1型细胞因子γ干扰素(IPN-γ)、白细胞介素-2(IL-2)、Th2型细胞因子白细胞介素-4(IL-4)、白细胞介素-10(IL-10)变化的规律：②上述细胞因子mRNA在T淋巴细胞和脾脏、肠系膜淋巴结(MLN)内表达的动态改变；③烧伤大鼠血清和焦痂提取物对离体大鼠T淋巴细胞Th1／Th2型细胞因子的变化规律的影响；④相应的T淋巴细胞转化率。探讨烧伤焦痂持续存在对机体免疫功能的危害，论证休克期切痂的必要性。 方法：清洁级雄性Wistar大鼠160只，随机分为四组：A组为单纯烧伤对照组；B、C、D组均为切痂组，分别于伤后8、24、96小时一次切除焦痂移植同种异体皮封闭创面，设立烧伤前、烧伤后4、12、24、48、96、120、168小时诸时相点，各时相点n=8。在各时相点处死动物，留取血液和脾脏、肠系膜淋巴结标本并即刻提取纯化T淋巴细胞，ELISA法检测IFN-γ、IL-4，放射免疫分析法检测IL-2、IL-10蛋白水平、RT-PCR方法半定量测定IFN-γ、IL-4 mRNA表达。以烧...
Objective: A 30% TBSA , full-thickness scald model of rats was employed to determine the type-l/type-2 cytokines response of helper T lymphocytes after thermal inhury and to investigate the effects of escharectomy during burn shock stage on the polarization of Th cells and host immune function. The mRNA and protein levels of interleukin(IL)-2, IL-4, IL-10 and interferon-gamma ( IFN-y ) and the proliferative response of T lymphocytes was also measured. In addition, in vito study was performed to observe the ... |