The characteristic is to insert a designed synthetic human gammar type interferon (IFN-gamma) group into colibacillus carrier containing gammar bacteriophage PL primary gene to build IFN-gamma expression particles, the IFN-gamma produced by the engineering bacteria containing the said particles reaches 60-80% of the total amount of bacteria soluble protein, the produced IFN-gamma are concentrated in the inclusion body, so that after separating the inclusion body, the purity of IFN-gamma reaches 90%, then through one step molecular sieve column chromatography, the purity can reach above 96%, the specific activity is 2X10 to the power 7 IU/mg.protein. |