A novel salmon calcitonin analog's gene msCT(31aa) is cloned by gene engineering and a gene expression system by fusing oleosin with target protein is created. Its steps includes separating and cloning the promotor of rape's oleosin gene, coding the structure gene of sesame's oleosin, designing the gene msCT for coding salmon calcitonin analog m3CT [Trp1, Ser6, Phe7 and del-Leu19], inserting it to C terminal of sesame oleosin gene, configuring the expression carrier of rape's oleosin gene promoter driven sesame oleosin-msCT fusion gene plant, transferring to rape and cotton by Agrobacterium method and pullen channel methods to respectively obtain their transgenic plants, and testing. It can be used to treat osteoporosis, Paget's disease, ostalgia, etc. |