A process for preparing novel micro-molecular mate by use of genetically engineered colibacillus includes such steps as activating the genetic engineering bacterial seeds in culture medium, culturing in fermented culture medium to accumulate the micro-molecular mate in the bacterial cells, centrifugal separating of bacterial cells, resuspending with buffer liquid, ultrasonic breaking of cells to release the micro-molecular mate into solution, centrifugal separating, collecting supernatant, affinity adsorbing by affinity chromatograph column, eluting, gel separating by gel column, and freeze drying at -40 deg.C. Its advantages are high effect, low cost and no environmental pollution. |