The invention relates to preparation of vaccine. Preparation of tick-borne encephalitis resisting vaccine is disclosed in the invention including the following steps. In the process to obtain vaccine, protein impurities are removed, especially proteins of chicken embryoincludes. Protamine sulfate which ultimate concentration is 50-100 mkg/ml is used to treat the inactivated virus suspension without preliminary purification, and remove the generated deposition and cell chips by continuous centrifuging and filtration. Concentration of the purificated culture medium by ultrafiltration. Purification of concentrated virus is performed due to chromatography upon porous silica. Chromatographically purified fraction is controlled for the content of total protein and proteins of chicken embryo, diluted with buffer solution in accordance to its antigenic activity to use in obtaining the sorbed vaccine. Concentration of proteins in chicken embryo in purified vaccine semifinished product does not exceed 1 mkg/ml vaccine dose. |