| Original document(13 pages) Authorized document(13 pages) 中文版 |
In the present invention an embryo or adult cornea epithelium is cut, digested with digesting liquid and centrifugated to prepare single cell suspension, and the cell suspension is cultured in culture dish or bottle with proper amount of culture medium and CO2 in 5% at 37 deg.c. The cultured cell is passed after cell converges to 80-90% and the second and sixth generation of cell is passed directly to amnion for culture for another 8-20 days to obtain the stem cell regenerating surface cornea of the present invention. The stem cell regenerating surface cornea may be used as material for treating corneal disease. The present invention provides a new material for treating corneal disease with rich material source, no danger of mouse-originated pollution, no or slight immunological rejection. |
Application Number
申请号 |
01123707 |
Application Date
申请日 |
2001.07.27 |
| Title 名称 |
Stem cell regenerating surface cornea and its application in corneal transplantation |
Publication Number
公开号 |
1398644 |
Publication Date
公开日 |
2003.02.26 |
| Approval Pub. Date |
|
Granted Pub. Date |
2005.09.14 |
| International Classification 分类号 |
A61L27/22,C12N5/06,A61F2/14 |
Applicant(s) Name
申请人 |
Keyu Union Stem Cell Biotechnology Co., Ltd., Beijing |
| Address 地址 |
100083 |
| Inventor(s) Name 发明人 |
Du Yiqin |
| Attorney & Agent 代理人 |
wang mengxia |
| More information 更 多 信 息 |
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