Original document(16 pages) Authorized document(16 pages) 中文版
    The present invention provides a high-sensitivity homogeneous DNA hibridization fluorescence determination method. Eu-compound of EDTA is used to label 5'-end or 3'-end DNA probe, a kind of didentate beta-diketone is used to label 5'-end or 3'-end DNA probe for simultaneous hibridization reaction. After two labeled DNA probes are hybridized with target DNA, EDTA-Eu and beta-diketone close to each other to form ternary strong fluorescence compound EDTA-Eu-diketone. By the time identifying fluorescence process, the fluorescence strength of the formed ternary fluorescence compound is measured and the concentration of the target DNa is determined. The present invention has high sensitivty and wide application.
Application Number
申请号		 01127967 Application Date
申请日		 2001.07.25
Title 名称 High-sensitivity homogeneous DNA hibridization fluorescence detection method
Publication Number
公开号		 1399126 Publication Date
公开日		 2003.02.26
Approval Pub. Date Granted Pub. Date 2005.06.22
International Classification 分类号 G01N21/64
Applicant(s) Name
申请人		 Dalian Inst. of Chemical Physics, Chinese Academy of Sciences
Address 地址 116023
Inventor(s) Name 发明人 Yuan Jingli
Attorney & Agent 代理人 xu zongfu zhou xiumei
More information 更  多  信  息


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