Original document(18 pages)  中文版
    A process for gene conversion of corn includes such steps as immersing the embryo or callus of corn in the agrobacterium D-inf solution containing acetyl syringone, waving for 30-60 seconds, laying aside for 5-60 min, adding it to D-As culture medium, culturing at 22-26 deg.c for 2-7 days in dark condition, washing with aseptic water containing thisporomycin, immersing for 15-60 min, culturing in the culture medium containing thisporomycin for 2-7 days, low-pressure selective culture, high-pressure selective culture, inducing embryoid, and differentiating in differential culture medium to obtain regenerated transgenic plant.
Application Number
申请号
02158758 Application Date
申请日
2002.12.26
Title 名称 Method for gene conversion of corn
Publication Number
公开号
1429904 Publication Date
公开日
2003.07.16
Approval Pub. Date Granted Pub. Date
International Classification 分类号 A01H1/00,A01H4/00,C12N5/00,C12N15/09,C12N15/87
Applicant(s) Name
申请人
China Agricultural Univ.
Address 地址 100094
Inventor(s) Name 发明人 Wang Guoying
Attorney & Agent 代理人 guan chang
More information 更  多  信  息


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