Original document(10 pages)  中文版
    A single embedded polyhedronvirus shuttle expression carrier of bollworm is configured through inserting the low-copy bacterium F replicon, kanamycine resistant gene and beta galactosidase gene to the polyhedrom gene site of the virus genom. The doner plasmid is used to transfer the exogenous gene to the insetion site of transposon in the said shuttle expression carrier. After bollworm cell is transfected by said DNA, the infections recombinant virus particles are formed to express exogenous gene under control of polyhedron promoter and P10 promoter.
Application Number
申请号		 01138377 Application Date
申请日		 2001.12.31
Title 名称 Cotton bollworm single particle embedded nuclear polyhedrosis virus shuttle expression carrier and preparation method
Publication Number
公开号		 1429910 Publication Date
公开日		 2003.07.16
Approval Pub. Date Granted Pub. Date
International Classification 分类号 A01N63/00,C12N7/01,C12N15/33,C12N15/79
Applicant(s) Name
申请人		 Wuhan Virus Inst., Chinese Academy of Sciences
Address 地址 430071
Inventor(s) Name 发明人 Wang Hanzhong, Chen Xinwen
Attorney & Agent 代理人 wang minfeng
More information 更  多  信  息


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