The present invention relates to the preparation of clastridium welchil toxoid vaccine and antitoxin serum. The preparation of clastridium welchil toxoid vaccine includes preparing toxoid via vibrating culture in culture medium of buffering liquid dissolved peptone, dextrin, yeast extracturm and L-arginine and under anerobic condition; deactivation, filtering and addition of adjuvant. The toxoid has determined and reliable active immunizing effect on animal's clastridium welchil infection. The antitoxin serum is obtained via several times of inoculation of the clastridium welchil toxoid vaccine and clastridium welchil vaccine to livestock. The antitoxin serum has complete neutralizing antibody potency, ideal passive immune effect and ideal treating effect on animal's clastridium welchil infection. |