The present invention relates to recombinant toxoplasma fusion protein, its preparation method and application. It utilizes gene engineering technology to prepare a new-type recombinant toxoplasma protein, i.e. the fusion protein formed from 110 amino acids from 43-th amino acid to 152-th amino acid of toxoplasma GRA6 protein N end and 258 amino acids from 56-th amino acid to 313-th amino acid of P30 protein which are series-connected. The above-mentioned 110 amino acids of GRA6 protein N end is positioned at fusion protein N end, and the 258 amino acids of P30 protein is positioned at C end of the fusion protein, and between them 2 amino acids of Gly and Ser are connected, and one methionine (Met) is added at N end of fusion protein, its total length has 371 amino acids. |