| Original document(10 pages) 中文版 |
A vinca rosea Glutathione S-transferase gene sequences, and it coding product amino acid sequence. It relates to a kind of new gene sequence, characterized in that: this gene is 929bp, at the point of 56bp there is the start coding triplet ATG, 718bp the ending coding triplet TGA, 910bp additional signal polyA. The gene sequence has not any inner gene, 663bp completely disclosed code-reading frame, code 220 amino acid. Nucleotide sequence and Capsicum Chinese gene GST1 has reached 67.7% homeotic source, and Euphorbia esula gene GST 57.7 homeotic source. This invention also provides a method to gain a full length of forced gene. First we enrich the anti-dry expressing gene in a moderate dry condition; then we establish full length genomic library of cDNA and detecting order, obtain the anti-dry related full length forcing gene in most short period. This anti-dry gene straightly links to eukaryotes expressing carrier pCMV plasmid, detected of the order by t3, t7 primer, filtered by eukaryotes expressing. This invention distills the full length gene of glucoside peptide transferase. This gene has a good anti-oxidate function, increases express in adverse circumstance forces in the process, the gene eliminates the free radical of oxidation. This gene colon expands the gene source of plant anti-dry, and provides a more rich and good usable gene for enhancing the anti-dry function and meliorating breed. |
Application Number
申请号 |
200510009937 |
Application Date
申请日 |
2005.04.27 |
| Title 名称 |
Gene sequence of glutathionetransferase of vinca rosea
|
Publication Number
公开号 |
1687422 |
Publication Date
公开日 |
2005.10.26 |
| Approval Pub. Date |
|
Granted Pub. Date |
|
| International Classification 分类号 |
C12N15/54,C12N9/10,C12N15/79 |
Applicant(s) Name
申请人 |
Northest Forestry Univ. |
| Address 地址 |
150040 |
| Inventor(s) Name 发明人 |
Fang Siliang, Guo Xiaorui, Jiang Yang, Nie Mingzhu, Tang Zhonghua, Wang Huimei, Yu Jinghua |
| Attorney & Agent 代理人 |
|
|
|