Original document(17 pages)  中文版
    The present invention provides method for E. coli to express lysostaphin in high effect via external secretion. The method includes cloning coding signal peptide suitable for secreting expression in E. coli in front of the gene sequence of partially or completely mature lysostaphin and connecting to promoter to constitute the expression vector; transforming the expression vector to E. coli and culturing fermentation; and separating lysostaphin from the supernatant of the fermented liquid. The external secretion expression has the advantages of the expression product existing in the culture medium in ultimate active form and without need of inclusion body renaturation and other steps; simple purification from culture medium supernatant and high recovering rate; and less host bacteria protein pollution.
Application Number
申请号		 200510028038 Application Date
申请日		 2005.07.22
Title 名称 Method for E, coli to express lysostaphin in high efficiency via external secretion
Publication Number
公开号		 1900290 Publication Date
公开日		 2007.01.24
Approval Pub. Date Granted Pub. Date
International Classification 分类号 C12N15/70;C12N9/36;C12N1/21
Applicant(s) Name
申请人		 Gaoke United Biological Technology R&D Co., Ltd., Shanghai
Address 地址
Inventor(s) Name 发明人 Huang Qingshan;Lu Hairong;Lu Wanying
Attorney & Agent 代理人 luo dachen

  
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