the invention discloses a hybridized carrier preparing method of high-activity solidified penicillin acylating enzyme, which comprises the following steps: blending cyclohexane, polyethylene glycol octylphenyl ether and positive hexyl alcohol evenly; adding ammonia; allocating transparent inverse-phase micro-emulsion; stirring for 15 min; adding silicic carbethoxy to stir for 30 min; adding siliconiting agent; setting the bulk rate of silicic carbethoxy and siliconiting agent at 0.5-9.5: 0.5-9.5; hydrolyzing; condensing for 22h; washing through acetone; drying in the vacuum at indoor temperature to obtain the product. |