the invention discloses a gene synthesizing method of equal-temperature single-direction growth in the gene engineering technological domain, which comprises the following steps: (1) designing to synthesize the extending oligonucleotide chain according to ordered DNA sequence; or purifying to synthesize oligonucleotide; (2) proceeding equal-temperature growth under multiple enzyme; synthesizing order DNA long-chain; (3) adopting the product in the step (2) as mold to proceed PCR augmentation; obtaining PCR product with ordered sequence length. |