the invention discloses a manufacturing method of rough rhamnolipid extract and application fermented by waste food and drink oil, which is characterized by the following: proceeding ultraviolet mutagenesis for natural sieved pseudomonas; picking fitful bacteria; adopting waste food and drink oil as ferment carbon source; making the density of rhamnolipid reach 35-45g/L; ultrafiltrating the ferment liquid to remove bacteria to obtain the powder-shaped rough extract through spraying and drying method; adding 0.01-3% rough extract in the biochemical dirty water to improve disposing efficiency. |