The invention provides arethusa mycorrhizal fungi separating culture key technology method which belongs to plant mycorrhizal fungi separatting culture technical field. The method includes the following steps: pre-processing for root; preparing single hypha body; then processing, sprouting, selecting by micromanipulation, and resetting to culture medium for it; checking it by microscope and resetting to plate purification for the survived. It can increase strain separatting reliability and reduce sieving workload. It is a key technology for arethusa mycorrhizal fungi further study and application development. |