The invention relates to causticity acid producing bacteria culturing method which belongs to new type causticity acid birthing bacteria producing culturing technology in corrosion and protection field. The separating purifying adopts aseptic processing includes the following steps: peeling off the biological film from copper nickel alloy surface dipped in sea for 180 days; enrichment culture by 2216E culture medium at room temperature whose enrichment solution are processed gradient dilution by 10-1, 10-2, 10-3, 10-4, then streaking at the flat; selecting the transparent colony to purify; repeating this to gain acid birthing bacteria pure culture; passage; selecting the strain after 50 times passage to store. The separating purifying method is simple, reliable, and safe. The cultured bacterium is pure and has better application effect. The cultured strain can be used in copper alloy corrosion accelerating process. |