The invention relates to Philippines little clam histiocyte culturing method. Its feature is that it includes the following steps: sterilizing germicidal treatment for tissue block that washing the tissue block by aseptic filtration sea water, then dipping it in sterilizing solution; inoculating that arranging it on the inner wall of the culture bottle at 26-27 degree centigrade for 4-6h; culturing that adding culture solution and culturing in constant temperature box at 26-27 degree centigrade pH 7.2-7.4. The advantages of the invention are that it not only can effectively exterminate the bacteria of the tissue block surface, but also can keep its activity to a greater extent to accelerate cell multiplication; and its operation is simple and easy. |