Original document(66 pages)  中文版
    The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1 alpha subunit of pPDH, the E1 beta subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1 alpha pPDH, E1 beta pPDH, E2pPDH, mtPDH or ALDH.
Application Number
申请号
200610100389 Application Date
申请日
1999.06.25
Title 名称 Materials and methods for the alteration of enzyme and acetyl coa levels in plants
Publication Number
公开号
1912118 Publication Date
公开日
2007.02.14
Approval Pub. Date Granted Pub. Date
International Classification 分类号 C12N15/09;C12N15/82;C12N15/52;C12N15/53;C12N15/54;C12N15/55;C12N15/60;C12N5/10;A01H5/00
Applicant(s) Name
申请人
Iowa State Universityres Found
Address 地址
Inventor(s) Name 发明人 Nikolau Basil J.;Wurtele Eve S.;Oliver David J.;Behal Robert;Schnable Patrick S.;Ke Jinshan;Johnson Jerry L.;Allred Carolyn C.;Fatland Beth;Lutziger Isabelle;Wen Tsui-jung
Attorney & Agent 代理人 tao jiarong
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