The invention discloses rice thread minute chromosome and its gaining method. It is made up of centromere, telomere, and one in three short arm of the rice number 11 chromosome. One rush of chain of the telomere DNA extends from 5'to 3', grows 7-10.5kb by connecting 1000-1500 repeating units TTTAGGG in series. Another one is extends from 3'to 5', grows 7-10.5kb by connecting 1000-1500 repeating units AAATCCC in series. Its gaining method is that it uses centromere special sequence RCS2 and/or the number 11 chromosome special sequence 5S as probe to filter 2n+1 type rice variant trisome contains double-centromere by fluorescence hybridization in situ. And it has great theoretical meaning and practical value for exploring the possibility of forming rice manpower chromosome. |