The invention discloses plant anther specificity promoter and its application. The promoter nucleotide sequence can be the one as (1) sequence table number one in question; or the one as (2), (1) in question nucleotide sequence complementary; or the one as (3), (1), or (2) in question which has 60% or above homology; or the one as (4), (1), (2), or (3) in question which can hybridized at strict hybridization condition. The inverse gene, small RNA, or expression vector can be used to transform host cell or tissue and its progeny cell to culture male sterile line plant, prevent transgene from polluting its wild species, landscaping plant, or crop species, etc. |