Original document(38 pages)  中文版
    The invention provides Escherichia coli expression vector and its using method. The expression vector is formed by vector pRSET-A whose ampicillin resistant gene is replaced by kanamycin resistant gene. And it can be used to express the enzyme needed in making 7-aminocephalosporanic acid but no beta-lactamase reduced its yield. The invention also provides two concrete expression vectors that pHS-GHA and pT7-kan-ACY whose gene products are respectively D-amino acid oxidase mutant GHA and Pseudomonas sp.SE83 glutaryl-7-aminocephalosporanic acid acidylating enzyme.
Application Number
申请号		 200510089966 Application Date
申请日		 2005.08.08
Title 名称 Colibacillus expression carrier and its application
Publication Number
公开号		 1912127 Publication Date
公开日		 2007.02.14
Approval Pub. Date Granted Pub. Date
International Classification 分类号 C12N15/70;C12N15/53;C12P21/02
Applicant(s) Name
申请人		 Bairui Globle Co., Ltd.
Address 地址
Inventor(s) Name 发明人 Wang Jun;Zeng Weiji;Ye Kangjian;Liu Zhaoming;Xiao Youlong;Zeng Shixian
Attorney & Agent 代理人 ding yebeng zhang tianshu
More information 更  多  信  息


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