The invention discloses RNA compatible medium and its manufacturing method used to separate and identify RNA singleness conjugated protein. It includes the following steps: fixing one end of DNA fragment of the plasmid pSP65 on glass dust of amino silane to form double chain DNA-glass dust; cloning target RNA gene or cDNA into another reciprocal clone site plasmid Psp64 to gain recombinant plasmid; gaining DNA fragment with extended nucleotide sequence by enzyme cutting; transcript RNA target sequence with RNA complementation fragment which is complemented with the plasmid pSP65 DNA fragment; heating the double chain DNA-glass dust and RNA target sequence together then cooling to make the RNA target sequence and signal chain DNA hybridize to form new RNA compatible medium which has the advantage of easy preparation, strong selectivity, repeatable utilization, etc. |