The invention relates to chip ligand and correlation technique used to identify protein sequence. It sifts and composes oligonucleotide proper gamete which can identify special tripeptide KAI, GEL, DGI, LAS as the protein chip ligand. The method used to identify protein sequence for the oligonucleotide proper gamete includes the following steps: using Structure-switch oligonucleotide proper gamete technique to approve that the oligonucleotide proper gamete can interact with the tripeptide sequence protein; biotin marking for the complex; fixing it on streptavidin labeled bead carrier; sampling, eluting, detecting for the protein; the composing of the four sequences can combine the protein contains the given four sequences. Thus the protein can be caught. |