The present invention belongs to the field of medicine technology, in the concrete, it relates to a freeze-dried powder injection preparation containing three vitamins of B group and its preparation method. It is characterized by that in the freeze-dried powder injection preparation a proper quantity of supporting agent (excipient) is added, the resolubility of said freeze-dried powder injection preparation can be effectively improved, and the chemical stability of vitamin B6 and vitamin B12 can be obviously raised. Said product can be used for preventing and curing several diseases of nerve injury, polyneuritis, prosopalgia, sciatica and others. |